Transfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction

نویسنده

  • ROLF BENZINGER
چکیده

Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. coli spheroplasts were plated with infertile rLT+ mLT+ Salmonella typhimurium indicator,

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Transfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction

Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. c...

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تاریخ انتشار 2003