Transfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction
نویسنده
چکیده
Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. coli spheroplasts were plated with infertile rLT+ mLT+ Salmonella typhimurium indicator,
منابع مشابه
Transfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction
Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. c...
متن کاملTransfection of Escherichia coli and Salmonella typhimurium spheroplasts: host-controlled restriction of infective bacteriophage P22 deoxyribonucleic acid.
Under proper conditions, one infective center was obtained for 3 x 10(8) molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coli were transfected in the presence of 25 mug of protamine sulfate per ml. A 3- to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected ...
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